il19 cytokine Search Results


mouse cxcl2 elisa kit  (Cloud-Clone corp)
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Cloud-Clone corp mouse cxcl2 elisa kit
Pro-inflammatory cytokine induction, autophagic flux impairment and NFKB activation by TcdB in human PMA-differentiated THP-1 macrophages. (A) Effects of exposure to TcdB (10 ng/ml) for the indicated time periods on the mRNA levels of IL1B, IL8 and <t>CXCL2</t> in cultured THP-1 macrophages. (B) Effects of exposure to TcdB at the indicated concentrations for 6 h on the mRNA levels of IL1B, IL8 , and CXCL2 in cultured THP-1 macrophages. LPS (100 ng/ml) was used as a positive control. (C) Exposing macrophages to TcdB (10 ng/ml) for 6 h decreased the protein levels of NFKBIA/IKB, enhanced NFKB RELA/p65 phosphorylation at serine 536, and promoted the nuclear translocation of NFKB RELA/p65 (green). Cytoplasmic and nuclear localization of NFKB RELA/p65 were visualized by immunofluorescence. Nuclei (blue) were labeled with DAPI. A total of 50 cells from each group were randomly selected for quantification by the ImageJ software. Scale bar: 5 µm (D) Exposing macrophages to TcdB (10 ng/ml) for 6 h led to increased protein levels of both LC3-II and SQSTM1/p62 (left panel). Exposing macrophages to TcdB (10 ng/ml) in the presence of bafilomycin A 1 (200 μM) for 6 h induced no further increase in SQSTM1/p62 protein levels (right panel). (E-F) Knockdown of SQSTM1 with siRNA abolished TcdB (10 ng/ml; 6 h)-induced (E) NFKB RELA/p65 phosphorylation at serine 536 and (F) mRNA expression of IL1B, IL8 , and CXCL2 . Protein and mRNA levels were quantified by Western blots and RT-qPCR, respectively. Results are expressed as mean ± S.E.M. from three independent experiments.
Mouse Cxcl2 Elisa Kit, supplied by Cloud-Clone corp, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Pro-inflammatory cytokine induction, autophagic flux impairment and NFKB activation by TcdB in human PMA-differentiated THP-1 macrophages. (A) Effects of exposure to TcdB (10 ng/ml) for the indicated time periods on the mRNA levels of IL1B, IL8 and CXCL2 in cultured THP-1 macrophages. (B) Effects of exposure to TcdB at the indicated concentrations for 6 h on the mRNA levels of IL1B, IL8 , and CXCL2 in cultured THP-1 macrophages. LPS (100 ng/ml) was used as a positive control. (C) Exposing macrophages to TcdB (10 ng/ml) for 6 h decreased the protein levels of NFKBIA/IKB, enhanced NFKB RELA/p65 phosphorylation at serine 536, and promoted the nuclear translocation of NFKB RELA/p65 (green). Cytoplasmic and nuclear localization of NFKB RELA/p65 were visualized by immunofluorescence. Nuclei (blue) were labeled with DAPI. A total of 50 cells from each group were randomly selected for quantification by the ImageJ software. Scale bar: 5 µm (D) Exposing macrophages to TcdB (10 ng/ml) for 6 h led to increased protein levels of both LC3-II and SQSTM1/p62 (left panel). Exposing macrophages to TcdB (10 ng/ml) in the presence of bafilomycin A 1 (200 μM) for 6 h induced no further increase in SQSTM1/p62 protein levels (right panel). (E-F) Knockdown of SQSTM1 with siRNA abolished TcdB (10 ng/ml; 6 h)-induced (E) NFKB RELA/p65 phosphorylation at serine 536 and (F) mRNA expression of IL1B, IL8 , and CXCL2 . Protein and mRNA levels were quantified by Western blots and RT-qPCR, respectively. Results are expressed as mean ± S.E.M. from three independent experiments.

Journal: Autophagy

Article Title: Vitamin D 3 and carbamazepine protect against Clostridioides difficile infection in mice by restoring macrophage lysosome acidification

doi: 10.1080/15548627.2021.2016004

Figure Lengend Snippet: Pro-inflammatory cytokine induction, autophagic flux impairment and NFKB activation by TcdB in human PMA-differentiated THP-1 macrophages. (A) Effects of exposure to TcdB (10 ng/ml) for the indicated time periods on the mRNA levels of IL1B, IL8 and CXCL2 in cultured THP-1 macrophages. (B) Effects of exposure to TcdB at the indicated concentrations for 6 h on the mRNA levels of IL1B, IL8 , and CXCL2 in cultured THP-1 macrophages. LPS (100 ng/ml) was used as a positive control. (C) Exposing macrophages to TcdB (10 ng/ml) for 6 h decreased the protein levels of NFKBIA/IKB, enhanced NFKB RELA/p65 phosphorylation at serine 536, and promoted the nuclear translocation of NFKB RELA/p65 (green). Cytoplasmic and nuclear localization of NFKB RELA/p65 were visualized by immunofluorescence. Nuclei (blue) were labeled with DAPI. A total of 50 cells from each group were randomly selected for quantification by the ImageJ software. Scale bar: 5 µm (D) Exposing macrophages to TcdB (10 ng/ml) for 6 h led to increased protein levels of both LC3-II and SQSTM1/p62 (left panel). Exposing macrophages to TcdB (10 ng/ml) in the presence of bafilomycin A 1 (200 μM) for 6 h induced no further increase in SQSTM1/p62 protein levels (right panel). (E-F) Knockdown of SQSTM1 with siRNA abolished TcdB (10 ng/ml; 6 h)-induced (E) NFKB RELA/p65 phosphorylation at serine 536 and (F) mRNA expression of IL1B, IL8 , and CXCL2 . Protein and mRNA levels were quantified by Western blots and RT-qPCR, respectively. Results are expressed as mean ± S.E.M. from three independent experiments.

Article Snippet: ELISA kits: TcdB (Mlbio, CDT-B), human IL1B (Wuhan Fine Biotech Co., EH0185), human IL8 (Cloud-clone corp, SEQ080Hu), human CXCL2 (C-X-C motif chemokine ligand 2; Cloud-clone corp, SEB603 Hu), mouse IL1B (Thermo Fisher Scientific, BMS6002), mouse IL8 (Wuhan Fine Biotech Co., EM1592), and mouse CXCL2 (Cloud-clone corp, SEB603Mu).

Techniques: Activation Assay, Cell Culture, Positive Control, Translocation Assay, Immunofluorescence, Labeling, Software, Knockdown, Expressing, Western Blot, Quantitative RT-PCR

Attenuation of Mitf downregulation, lysosome dysfunction, and pro-inflammatory cytokine expression in colonic macrophages by vitamin D 3 and carbamazepine in the murine CDI model. (A) Workflow of isolation of colonic macrophages is shown. (B-C) Effects of CDI without or with pre-treatment of (B) vitamin D 3 (VD3; 2300 IU/kg by oral gavage) and (C) carbamazepine (CBZ; 50 mg/kg by oral gavage) every other day for 2 weeks on mRNA expression of MITF and the four lysosomal proton pump subunits in isolated colonic macrophages are shown (pooled from three batches of experiments; BHI group, n = 9; C. difficile group, n = 9; CBZ or VD3 group, n = 6 each; C. difficile with CBZ or VD3 group, n = 9 each). (D) Effects of CDI without or with pre-treatment of vitamin D 3 or carbamazepine on mean fluorescence intensity (MFI) of LysoTracker Red staining in isolated colonic macrophages are shown. (E-F) Effects of CDI without or with pre-treatment of (E) vitamin D 3 and (F) carbamazepine on mRNA expression of Il1b, Il8 and Cxcl2 in isolated colonic macrophages are shown.

Journal: Autophagy

Article Title: Vitamin D 3 and carbamazepine protect against Clostridioides difficile infection in mice by restoring macrophage lysosome acidification

doi: 10.1080/15548627.2021.2016004

Figure Lengend Snippet: Attenuation of Mitf downregulation, lysosome dysfunction, and pro-inflammatory cytokine expression in colonic macrophages by vitamin D 3 and carbamazepine in the murine CDI model. (A) Workflow of isolation of colonic macrophages is shown. (B-C) Effects of CDI without or with pre-treatment of (B) vitamin D 3 (VD3; 2300 IU/kg by oral gavage) and (C) carbamazepine (CBZ; 50 mg/kg by oral gavage) every other day for 2 weeks on mRNA expression of MITF and the four lysosomal proton pump subunits in isolated colonic macrophages are shown (pooled from three batches of experiments; BHI group, n = 9; C. difficile group, n = 9; CBZ or VD3 group, n = 6 each; C. difficile with CBZ or VD3 group, n = 9 each). (D) Effects of CDI without or with pre-treatment of vitamin D 3 or carbamazepine on mean fluorescence intensity (MFI) of LysoTracker Red staining in isolated colonic macrophages are shown. (E-F) Effects of CDI without or with pre-treatment of (E) vitamin D 3 and (F) carbamazepine on mRNA expression of Il1b, Il8 and Cxcl2 in isolated colonic macrophages are shown.

Article Snippet: ELISA kits: TcdB (Mlbio, CDT-B), human IL1B (Wuhan Fine Biotech Co., EH0185), human IL8 (Cloud-clone corp, SEQ080Hu), human CXCL2 (C-X-C motif chemokine ligand 2; Cloud-clone corp, SEB603 Hu), mouse IL1B (Thermo Fisher Scientific, BMS6002), mouse IL8 (Wuhan Fine Biotech Co., EM1592), and mouse CXCL2 (Cloud-clone corp, SEB603Mu).

Techniques: Expressing, Isolation, Fluorescence, Staining

Attenuation of Mitf downregulation, lysosome dysfunction, and pro-inflammatory cytokine expression in colonic macrophages by vitamin D 3 and carbamazepine in the murine colon loop ligation model. (A) Workflow of pre-treatment with lysosome activators in the colon loop ligation model is shown. (B) A schematic illustration of the colon loop ligation model. (C) Histopathological scores of sealed colon segment tissues. Scale bar: 200 µm (D-E) mRNA expression of Mitf and the four lysosomal proton pump subunits in isolated colonic macrophages are shown. (F) Effects of TcdB without or with pre-treatment of vitamin D 3 (VD3) or carbamazepine (CBZ) on mean fluorescence intensity (MFI) of LysoTracker Red staining in isolated colonic macrophages are shown. (G) Effects of TcdB without or with pre-treatment with vitamin D 3 or carbamazepine on mRNA expression of Il1b, Il8 and Cxcl2 in isolated colonic macrophages are shown. **, p < 0.01; ***, p < 0.001 significantly different between indicated groups (pooled from three batches of experiments; Sham group, n = 6; TcdB group, n = 6; TcdB with CBZ or VD3 group, n = 6 each).

Journal: Autophagy

Article Title: Vitamin D 3 and carbamazepine protect against Clostridioides difficile infection in mice by restoring macrophage lysosome acidification

doi: 10.1080/15548627.2021.2016004

Figure Lengend Snippet: Attenuation of Mitf downregulation, lysosome dysfunction, and pro-inflammatory cytokine expression in colonic macrophages by vitamin D 3 and carbamazepine in the murine colon loop ligation model. (A) Workflow of pre-treatment with lysosome activators in the colon loop ligation model is shown. (B) A schematic illustration of the colon loop ligation model. (C) Histopathological scores of sealed colon segment tissues. Scale bar: 200 µm (D-E) mRNA expression of Mitf and the four lysosomal proton pump subunits in isolated colonic macrophages are shown. (F) Effects of TcdB without or with pre-treatment of vitamin D 3 (VD3) or carbamazepine (CBZ) on mean fluorescence intensity (MFI) of LysoTracker Red staining in isolated colonic macrophages are shown. (G) Effects of TcdB without or with pre-treatment with vitamin D 3 or carbamazepine on mRNA expression of Il1b, Il8 and Cxcl2 in isolated colonic macrophages are shown. **, p < 0.01; ***, p < 0.001 significantly different between indicated groups (pooled from three batches of experiments; Sham group, n = 6; TcdB group, n = 6; TcdB with CBZ or VD3 group, n = 6 each).

Article Snippet: ELISA kits: TcdB (Mlbio, CDT-B), human IL1B (Wuhan Fine Biotech Co., EH0185), human IL8 (Cloud-clone corp, SEQ080Hu), human CXCL2 (C-X-C motif chemokine ligand 2; Cloud-clone corp, SEB603 Hu), mouse IL1B (Thermo Fisher Scientific, BMS6002), mouse IL8 (Wuhan Fine Biotech Co., EM1592), and mouse CXCL2 (Cloud-clone corp, SEB603Mu).

Techniques: Expressing, Ligation, Isolation, Fluorescence, Staining